Stormwater May 2012 : Page 25

runoff. This location was chosen Samples Duration of Deployment Date Collection Date Analyses Comments as representative of the most Collected Biofilm Growth common environment within the March 4, 2009 Coupons were lost Enterococcus March 11, 2009 1 week Surrounding water during a small storm quantification municipal separate storm sewer event. Upstream biofilms system (MS4), where pipes are Coupons deployed. April 9, 2009 April 23, 2009 2 weeks Enterococcus enclosed and constantly wetted. Significant sediment quantification buildup was observed Downstream The second sample loca-around coupons. biofilms tion was downstream, within an May 6, 2009 4 weeks Enterococcus Some trash and sedi-quantification and ment buildup were open concrete channel exposed Sediments enterococcus observed around to sunlight and a constant ﬂ ow speciation coupons. MS4 surface of water from the upstream Fine sediments, leaf May 18, 2009 6 weeks Enterococcus biofilms litter, and organics quantification sample location (Figure 4). The were observed around coupons. downstream sample location was Early biofilm Enterococcus February 5, 2010 February 17, 2010 2 weeks Biofilms and chosen as representative of com-development quantification surrounding water March 2, 2010 4 weeks monly constructed open con-reassessment crete storm drain channels. March 31, 2010 8 weeks Post-storm sample The coupon deployment and April 8, 2010 10 weeks Post-storm sample retrieval schedule is presented in Table 1. Immediately after har-vesting, coupons were placed in 100 mL of phosphate buf-9230B). After four weeks of growth, isolates of enterococci fer saline (PBS) solution and were sonicated in the ﬁ eld to from sediments, surrounding water, and bioﬁ lms from the remove bioﬁ lm from only the lower surface of each coupon. concrete coupons were identiﬁ ed to a species level using In addition to coupon retrieval, naturally occurring bioﬁ lms the VITEK 2 Compact Identiﬁ cation System (bioMérieux attached to sediments and plants were also collected for as-Inc.). VITEK 2 Gram Positive Identiﬁ cation cards are used sessment of enterococci presence. All samples were placed in conjunction with the VITEK 2 Compact system for the on ice, transported to the laboratory, and enumerated for automated identiﬁ cation of microorganisms. The organisms enterococci using multiple tube fermentation (Method SM identiﬁ ed are dependent upon the cards utilized. Table 1. Early Biofilm Development—Deployment and Harvesting of Coupons in Tecolote Creek Is there something missing from your stormwater treatment toolkit? adsorption media may be the solution you’re looking for. Introducing APTsorb, a porous granular media effective in the adsorption of dissolved heavy metals. The patent-pending thermal and chemical treatment processes have been in development since 2004. These processes produce an active media with increased cationic capacity. APTsorb has high granular strength allowing for increased hydraulic loading. APTsorb performs well in standard sand ½OWHU©HTXLSPHQW&#0f;©DV©ZHOO©DV©HQJLQHHUHG©V\VWHPV APTsorb works to adsorb many dissolved heavy metals such as: Cu, Ni, Pb, Cd, Cr, Zn, Al, Co, and Mn American Peat Technology LLC 36203 350th Ave Aitkin MN, 56431 218.927.7888 info@americanpeattech.com www.AmericanPeatTech.com APTsorb may be the solution you are missing in your toolkit of stormwater treatment solutions. Contact us to learn more about APTsorb and what it can do for you. May 2012 www.stormh2o.com 25